| Citation: |
Jing Li, Chang-ling Liu, Neng-you Wu, Xiao-qing Xu, Gao-wei Hu, Yan-long Li, Qing-guo Meng, 2022. Identification of functionally active aerobic methanotrophs and their methane oxidation potential in sediments from the Shenhu Area, South China Sea, China Geology, 5, 285-292. doi: 10.31035/cg2022011
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Identification of functionally active aerobic methanotrophs and their methane oxidation potential in sediments from the Shenhu Area, South China Sea
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Abstract
Large amounts of gas hydrate are distributed in the northern slope of the South China Sea, which is a potential threat of methane leakage. Aerobic methane oxidation by methanotrophs, significant methane biotransformation that occurs in sediment surface and water column, can effectively reduce atmospheric emission of hydrate-decomposed methane. To identify active aerobic methanotrophs and their methane oxidation potential in sediments from the Shenhu Area in the South China Sea, multi-day enrichment incubations were conducted in this study. The results show that the methane oxidation rates in the studied sediments were 2.03‒2.36 μmol/gdw/d, which were higher than those obtained by sediment incubations from other areas in marine ecosystems. Thus the authors suspect that the methane oxidation potential of methanotrophs was relatively higher in sediments from the Shenhu Area. After the incubations family Methylococcaea (type I methanotrophs) mainly consisted of genus Methylobacter and Methylococcaea_Other were predominant with an increased proportion of 70.3%, whereas Methylocaldum decreased simultaneously in the incubated sediments. Collectively, this study may help to gain a better understanding of the methane biotransformation in the Shenhu Area.
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References
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Jing Li, Chang-ling Liu, Neng-you Wu, Xiao-qing Xu, Gao-wei Hu, Yan-long Li, Qing-guo Meng, 2022. Identification of functionally active aerobic methanotrophs and their methane oxidation potential in sediments from the Shenhu Area, South China Sea, China Geology, 5, 285-292. doi: 10.31035/cg2022011
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Figure 1.
Experimental culture system in this study.
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Figure 2.
Methane consumption by methanotrophs over the incubation time with NH_1 and NH_2. Linear regression of the steepest curves showed in at image top right.
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Figure 3.
Distribution of FAMEs in studied sediment samples (m/z=74, internal standard). a‒NH_1_Original; b‒NH_1_Incubated_45days; c‒NH_2_Original; d‒NH_2_Incubated_45days.
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Figure 4.
pmoA gene copy number and CH4 content by methanotrophs over the incubation time of NH_1.
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Figure 5.
Taxonomic composition of methanotrophic communities in studied sediment samples.