Simultaneous Determination of Chromium(Ⅲ) and Chromium(Ⅵ) in Water by the First Derivative Spectrophotometric Method

Hong-na XU; Li-guo JIN; Li-mei YOU; Yan CHENG

doi:10.15898/j.cnki.11-2131/td.201908090118

2020 Vol. 39, No. 5

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Hong-na XU, Li-guo JIN, Li-mei YOU, Yan CHENG. Simultaneous Determination of Chromium(Ⅲ) and Chromium(Ⅵ) in Water by the First Derivative Spectrophotometric Method[J]. Rock and Mineral Analysis, 2020, 39(5): 785-792. doi: 10.15898/j.cnki.11-2131/td.201908090118

Citation:

Hong-na XU, Li-guo JIN, Li-mei YOU, Yan CHENG. Simultaneous Determination of Chromium(Ⅲ) and Chromium(Ⅵ) in Water by the First Derivative Spectrophotometric Method[J]. Rock and Mineral Analysis, 2020, 39(5): 785-792. doi: 10.15898/j.cnki.11-2131/td.201908090118

Simultaneous Determination of Chromium(Ⅲ) and Chromium(Ⅵ) in Water by the First Derivative Spectrophotometric Method

1.
Mudanjiang Medical University, Mudanjiang 157011, China
2.
The First Geological Prospecting Institute of Heilongjiang Province, Mudanjiang 157011, China

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Corresponding author: Li-guo JIN, yaoguo97@163.com

Received Date: 09 August 2019

Revised Date: 11 December 2019

Accepted Date: 16 April 2020

Abstract

Abstract

OBJECTIVES Chromium is usually found as Cr(Ⅲ) and Cr(Ⅵ) in environmental water samples. The different valences of chromium produce different physiological function, thus it is necessary to analyze the different valences of chromium accurately. At present, the contents of Cr(Ⅲ) and Cr(Ⅵ) are mostly determined after separation or the content of Cr(Ⅲ) or Cr(Ⅵ) is measured first, then the total content of Cr is determined after oxidation or reduction. The content of another valence of chromium is then calculated by the subtraction method. The available method needs a complex procedure. Moreover, the valence of Cr is easily modified during the sample treatment, resulting in large error and low precision. OBJECTIVES To find a simple and accurate method for determination of Cr(Ⅲ) and Cr(Ⅵ). METHODS The first derivative spectrophotometric method was used for simultaneously determining of Cr(Ⅲ) and Cr(Ⅵ), which eliminated the interference of Cr(Ⅲ) on Cr(Ⅵ). RESULTS Chromogenic reagent EDTA-2Na was added to the mixed water sample in a 70℃water-bath at pH 3-3.5 for 15min, and the absorbance was measured. When the derivative interval factor was 10nm, the first derivative value of absorption for Cr(Ⅵ) was the maximum at 330nm wavelength and the value for Cr(Ⅲ)-EDTA was zero. The concentration of Cr(Ⅵ) can be obtained by the first derivative spectrophotometric method, whereas Cr(Ⅲ) can be determined directly at the maximum wavelength of 543nm. Under the optimal conditions, the concentration range was 0-100mg/L for Cr(Ⅵ) and 0-120mg/L for Cr(Ⅲ). The equation of linear regression for Cr(Ⅲ) was A=0.0036ρ-0.0002 (r²=0.9999), for Cr(Ⅵ) was D=0.00072ρ-0.00013 (r²=0.9996). and the limit of detection was 0.005mg/L for Cr(Ⅵ) and 0.006mg/L for Cr(Ⅲ). The recoveries for Cr(Ⅲ) and Cr(Ⅵ) were 97.8%-102.6%. Conclusion The method meets the requirements for analyzing waste water.
- water sample /
- Cr(Ⅲ) /
- Cr(Ⅵ) /
- simultaneous determination /
- derivative interval factor /
- first derivative spectrophotometry

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References

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