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2021 No. 5
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ZHAO Jia-yi, ZHEN Shi-jun, ZHANG Cui-yun, YIN Mi-ying, ZHANG Sheng. 2021. Composition and Functional Prediction of Microbial Communities in Deep Geothermal Water from Jizhong (Central Hebei) Geothermal Area. Acta Geoscientica Sinica, (5): 605-616. doi: 10.3975/cagsb.2021.042601
Citation: ZHAO Jia-yi, ZHEN Shi-jun, ZHANG Cui-yun, YIN Mi-ying, ZHANG Sheng. 2021. Composition and Functional Prediction of Microbial Communities in Deep Geothermal Water from Jizhong (Central Hebei) Geothermal Area. Acta Geoscientica Sinica, (5): 605-616. doi: 10.3975/cagsb.2021.042601
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Composition and Functional Prediction of Microbial Communities in Deep Geothermal Water from Jizhong (Central Hebei) Geothermal Area

  • 1. Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences, Shijiazhuang, Hebei 050061;

  • 2. Key Laboratory of Groundwater Remediation of Hebei Province and China Geological Survey, Shijiazhuang, Hebei 050061

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  • Corresponding author: ZHANG Cui-yun  
Received Date:  24 November 2020
Revised Date:  06 April 2021
    Abstract
  • The understanding of the composition and functions of deep microbial communities is of great significance for exploring the origin of early lives and utilizing their unique functions.The composition and functions of microbial communities in deep carbonate karst-fissure geothermal storage environment remain unclear.In order to explore the composition and prediction of the functions of microbial communities in the deep geothermal water from the deep carbonate rock karst-fissure geothermal storage environment, the authors, taking shallow water and soil as the references, collected deep geothermal water samples by the pumping tests using the geothermal scientific drilling holes located in Jizhong geothermal area and also collected shallow water samples from farm irrigation wells and soil samples near the top of each drill hole, respectively for 16S rRNA gene high-throughput sequencing and functional predictions using PICRUSt.The results show that there exist 38 phyla and 541 genera in the deep geothermal water of Jizhong geothermal area, of which bacteria are dominant (97.5%), whereas archaea are rare (2.5%), and characteristic microbial communities comprise mainly Firmicutes, Nitrospirae, Thermotogae and Euryarchaeota.The dominant bacterial genera include sulfate-reducing bacteria.Carbon fixation, fermentation and sulfate reduction might be relatively strong, whereas methanogenesis and denitrification might be weak in the deep geothermal water.Aerobic, mesophilic bacteria occurring in the deep geothermal water indicate that the deep geothermal water is recharged by the shallow water rich in proteobacteria under the condition of large flow pumping.This study has revealed that the deep carbonate rock karst-fracture geothermal reservoirs contain abundant microbial communities with various functions in Jizhong geothermal area, and that the characteristics of microbial communities can be used as indicators of the hydraulic connection between deep geothermal water and shallow water.
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