| Professional Committee of Rock and Mineral Testing Technology of the Geological Society of China, National Geological Experiment and Testing Center | Host |
| Citation: |
WANG Junjie, WANG Qiaohuan, XIONG Manyan, SONG Xiangmei, WANG Meie, FU Huimin, LI Hong, MENG Ling. Determination of Organic Carbon in Soil and Sediment by the Elemental Analyzer with Silver Cup Digestion Method[J]. Rock and Mineral Analysis, 2024, 43(6): 936-944. doi: 10.15898/j.ykcs.202409100185
|
Determination of Organic Carbon in Soil and Sediment by the Elemental Analyzer with Silver Cup Digestion Method
-
Abstract
Soil organic carbon (SOC) is an important indicator of soil health and ecosystem functioning and is of great significance for agricultural production and environmental protection. Accurate determination of SOC content is essential for assessing soil quality, optimizing fertilizer management, improving crop yields, and contributing greatly in the research of tackling climate change as well as the carbon cycle. A rapid and accurate method for the determination of organic carbon in soil and sediment was established to support scientific research and agricultural production. The silver cup digestion-elemental analyzer method was used to process soil samples using 81-porosity copper plates and silver cups by optimizing sample pretreatment, including acid concentration and volume, sample weight as well as digestion temperature. The specific procedure was initially weighing 25mg of sample using Φ5mm×9mm silver cups, then adding 80L of 2mol/L hydrochloric acid to remove inorganic carbon, followed by drying the sample on an electric hot plate at 70℃. Samples with high inorganic carbon were treated by repeated acid addition. Low and high content standards were used to form a mixed standard curve to quantify the organic carbon with a daily calibration method. The method demonstrated excellent linearity in the range of 21.2−6704.3μg of organic carbon content with a linear correlation coefficient of 0.9998. The limits of detection (LOD) and quantification (LOQ) of the method were 0.07% and 0.23%, respectively, based on a sample weight of 25mg. The precision of organic carbon determined for six standard substances (GBW07406, GBW07385, GBW07391, GBW07544, GBW07544, GBW07546 and GBW07365) ranged from 0.67% to 5.14%, with all measured values falling within the range of the standard values. The method demonstrates high precision and accuracy, which meets the requirements for the determination of organic carbon in soil and sediment and is suitable for the analysis of large quantities of samples.
-
Keywords:
- soil /
- sediments /
- organic carbon /
- silver cup digestion /
- element analyzer
-
-
References
[1] 查同刚. 土壤理化分析[M]. 北京: 中国林业出版社, 2017: 79−87. Zha T G. Soil physicochemical analysis[M]. Beijing: China Forestry Publishing House, 2017: 79−87 [2] Hicks P C E, Ryals R, Zhu B, et al. The deep soil organic carbon response to global change[J]. Annual Review of Ecology, Evolution, and Systematics, 2023, 54(1): 375−401. doi: 10.1146/annurev-ecolsys-102320-085332 [3] Ferraz M A, Choueri R B, Castro Í B, et al. Influence of sediment organic carbon on toxicity depends on organism’s trophic ecology[J]. Environmental Pollution, 2020, 261: 114134.1−114134.10. doi: 10.1016/j.envpol.2020.114134 [4] 李源, 尹肖尧, 常钟元. 高频红外碳硫仪测定土壤和沉积物中有机质[J]. 化学分析计量, 2020, 29(6): 75−78. doi: 10.3969/j.issn.1008-6145.2020.06.017 Li Y, Yin X Y, Chang Z Y. Determination of organic matter in soil and sediment by high frequency infrared carbon sulphur instrument[J]. Chemical Analysis and Meterage, 2020, 29(6): 75−78. doi: 10.3969/j.issn.1008-6145.2020.06.017 [5] 吴才武, 夏建新, 段峥嵘. 土壤有机质测定方法述评与展望[J]. 土壤, 2015, 47(3): 453−460. doi: 10.13758/j.cnki.tr.2015.03.004 Wu C W, Xia J X, Duan Z R. Review on detection methods of soil organic matter (SOM)[J]. Soils, 2015, 47(3): 453−460. doi: 10.13758/j.cnki.tr.2015.03.004 [6] 李国傲, 何咏, 陈雪, 等. 全自动消解测定土壤/沉积物中的有机碳[J]. 环境工程, 2016, 34(5): 152−155. doi: 10.13205/j.hjgc.201605033 Li G A, He Y, Chen X, et al. Determina of organic carbon in soil/sediments by automatic digestion[J]. Environmental Engineering, 2016, 34(5): 152−155. doi: 10.13205/j.hjgc.201605033 [7] 李朝英, 郑路, 郑之卓, 等. 自动滴定仪测定土壤有机碳及其组分的方法优化[J]. 岩矿测试, 2024, 43(4): 632−640. doi: 10.15898/j.ykcs.202404210092 Li Z Y, Zheng L, Zheng Z Z, et al. Method optimization for the determination of soil organic carbon and its components by automatic titrator[J]. Rock and Mineral Analysis, 2024, 43(4): 632−640. doi: 10.15898/j.ykcs.202404210092 [8] 杨林婧, 杨莎, 张圣杨, 等. 农田土壤有机碳高光谱特征及定量监测研究[J]. 激光生物学报, 2024, 33(4): 316−325. doi: 10.3969/j.issn.1007-7146.2024.04.004 Yang L J, Yang S, Zhang S Y, et al. Hyperspectral characteristics and quantitative monitoring of soil organic carbon in farmland[J]. Acta Laser Biology Sinica, 2024, 33(4): 316−325. doi: 10.3969/j.issn.1007-7146.2024.04.004 [9] 于雯泉, 钟少军. 海洋沉积物有机碳分析方法中干燥预处理过程人为误差的发现及其意义[J]. 环境科学学报, 2007, 27(5): 861−867. doi: 10.3321/j.issn:0253-2468.2007.05.025 Yu W Q, Zhong S J. Freeze-drying pretreatment improves organic carbon determinations of marine sediments[J]. Acta Scientiac Circumstantiac, 2007, 27(5): 861−867. doi: 10.3321/j.issn:0253-2468.2007.05.025 [10] 高少鹏, 徐柏青, 王君波, 等. 总有机碳分析仪准确测定湖泊沉积物中的TOC[J]. 分析试验室, 2019, 38(4): 413−416. doi: 10.13595/j.cnki.issn1000-0720.2018.072501 Gao S P, Xu B Q, Wang J B, et al. Measuring total organic carbon precisely in lake sediment in Tibetan Plateau by TOC analyzer[J]. Chinese Journal of Analysis Laboratory, 2019, 38(4): 413−416. doi: 10.13595/j.cnki.issn1000-0720.2018.072501 [11] 秦超, 李飞, 李贺, 等. 高频红外碳硫仪测定土壤中低含量的碳, 硫, 有机碳[J]. 当代化工研究, 2022(8): 47−49. doi: 10.3969/j.issn.1672-8114.2022.08.015 Qin C, Li F, Li H, et al. Determination of low content of carbon, sulfur and organic carbon in soil by high frequency infrared carbon and sulfur analyzer[J]. Modern Chemical Research, 2022(8): 47−49. doi: 10.3969/j.issn.1672-8114.2022.08.015 [12] Avramidis P, Bekiari V. Application of a catalytic oxidation method for the simultaneous determination of total organic carbon and total nitrogen in marine sediments and soils[J]. PLos One, 2021, 16(6): e0252308. doi: 10.1371/journal.pone.0252308 [13] Hu J, Loh P S, Chen Z, et al. Storage and dynamics of soil organic carbon in allochthonous-dominated and nitrogen-limited natural and planted mangrove forests in Southern Thailand[J]. Marine Pollution Bulletin, 2024, 200: 1.1−1.14. doi: 10.1016/j.marpolbul.2024.116064 [14] Ma H, Peng M, Yang Z, et al. Spatial distribution and driving factors of soil organic carbon in the Northeast China Plain: Insights from latest monitoring data[J]. Science of the Total Environment, 2024, 911: 168602. doi: 10.1016/j.scitotenv.2023.168602 [15] Hernández T D B, Slater B K, Shaffer J M, et al. Comparison of methods for determining organic carbon content of urban soils in Central Ohio[J]. Geoderma Regional, 2023, 34: e00680. doi: 10.1016/j.geodrs.2023.e00680 [16] 王巧环, 任玉芬, 孟龄, 等. 元素分析仪同时测定土壤中全氮和有机碳[J]. 分析试验室, 2013, 32(10): 41−45. Wang Q H, Ran Y F, Meng L, et al. Simultaneous determination of total nitrogen and organic carbon in soil with an elemental analyzer[J]. Chinese Journal of Analysis Laboratory, 2013, 32(10): 41−45. [17] 谭扬, 吴学丽, 侯立杰. 样品处理方法对海洋沉积物有机碳稳定同位素测定的影响[J]. 海洋环境科学, 2018, 37(5): 780−784. doi: 10.13634/j.cnki.mes.2018.05.023 Tan Y, Wu X L, Hou L J. The effects of sample treatment methods on marine sediment organic carbon stable isotope[J]. Marine Environmental Science, 2018, 37(5): 780−784. doi: 10.13634/j.cnki.mes.2018.05.023 [18] 祝孟博, 宋建中, 彭平安. 预处理过程对不同类型样品中有机碳含量和稳定碳同位素测定的影响[J]. 地球与环境, 2015, 43(4): 476−482. doi: 10.14050/j.cnki.1672-9250.2015.04.015 Zhu M B, Song J Z, Peng P A. Influences of pretreatment procedures on determination of total organic carbon and stable carbon isotope in different samples[J]. Earth and Environment, 2015, 43(4): 476−482. doi: 10.14050/j.cnki.1672-9250.2015.04.015 [19] 闵秀云, 武君, 高春亮, 等. 基于元素分析仪测定土壤有机碳的不同前处理方法对比研究[J]. 盐湖研究, 2020, 28(4): 64−70. doi: 10.12119/j.yhyj.202004008 Min X Y, Wu J, Gao C L, et al. The comparative study on different pretreatment methods of soil organic carbon determined by elemental analyzer[J]. Journal of Salt Lake Research, 2020, 28(4): 64−70. doi: 10.12119/j.yhyj.202004008 [20] 孙萱, 宋金明, 于颖, 等. 元素分析仪快速测定海洋沉积物TOC和TN的条件优化[J]. 海洋科学, 2014, 38(7): 14−19. doi: 10.11759/hykx20130801001 Sun X, Song J M, Yu Y, et al. A rapid method for determing the total organic carbon and total nitrogen in marine sediments with an elemental analyzer[J]. Marine Sciences, 2014, 38(7): 14−19. doi: 10.11759/hykx20130801001 [21] Apesteguia M, Plante A F, Virto I. Methods assessment for organic and inorganic carbon quantification in calcareous soils of the Mediterranean region[J]. Geoderma Regional, 2018, 12: 39−48. doi: 10.1016/j.geodrs.2017.12.001 [22] Matsui Y, Fujisaki W, Torimoto J, et al. Gold-coated silver capsule for elemental analyzer-isotope ratio mass spectrometer: Robust against pretreatment of rock material for organic carbon and δ13C analyses[J]. Geochemical Journal, 2021, 55(3): e1−e8. doi: 10.2343/geochemj.2.0626 [23] 孙辞, 崔杰华, 林桂凤, 等. 元素分析仪测定低含碳量土壤样品方法的改进[J]. 辽宁大学学报: 自然科学版, 2017, 44(4): 337−341. doi: 10.16197/j.cnki.lnunse.2017.04.010 Sun C, Cui J H, Lin G F, et al. Improvement of methodology for determining low carbon soil samples by elemental analyzer[J]. Journal of Liaoning University: Natural Sciences Edition, 2017, 44(4): 337−341. doi: 10.16197/j.cnki.lnunse.2017.04.010 -
Access History
Figures(4)
Tables(6)
Export File
Citation
WANG Junjie, WANG Qiaohuan, XIONG Manyan, SONG Xiangmei, WANG Meie, FU Huimin, LI Hong, MENG Ling. Determination of Organic Carbon in Soil and Sediment by the Elemental Analyzer with Silver Cup Digestion Method[J]. Rock and Mineral Analysis, 2024, 43(6): 936-944. doi: 10.15898/j.ykcs.202409100185
Format
Content
DownLoad:
-
Figure 1.
Pretreatment process of organic carbon analysis in soil
-
Figure 2.
Effect of hydrochloric acid concentration on the determination of organic carbon
-
Figure 3.
Effect of hydrochloric acid addion volume on the determination of organic carbon.
-
Figure 4.
Effect of digestion numbers of the samples with high inorganic carbon content on the determination of organic carbon