Optimization of Extraction Method for Easily Extractable Glomalin-Related Soil Protein from Calcareous Soil

JIANG Li; KANG Zhiqiang; LIANG Yueming; WU Zeyan; TU Chun; LI Qiang

doi:10.15898/j.ykcs.202402060015

2024 Vol. 43, No. 4

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JIANG Li, KANG Zhiqiang, LIANG Yueming, WU Zeyan, TU Chun, LI Qiang. Optimization of Extraction Method for Easily Extractable Glomalin-Related Soil Protein from Calcareous Soil[J]. Rock and Mineral Analysis, 2024, 43(4): 582-591. doi: 10.15898/j.ykcs.202402060015

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JIANG Li, KANG Zhiqiang, LIANG Yueming, WU Zeyan, TU Chun, LI Qiang. Optimization of Extraction Method for Easily Extractable Glomalin-Related Soil Protein from Calcareous Soil[J]. Rock and Mineral Analysis, 2024, 43(4): 582-591. doi: 10.15898/j.ykcs.202402060015

Optimization of Extraction Method for Easily Extractable Glomalin-Related Soil Protein from Calcareous Soil

1.
Environmental Science and Engineering College, Guilin University of Technology, Guilin 541004, China
2.
Key Laboratory of Karst Dynamics, Ministry of Natural Resources and Guangxi, Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin 541004, China
3.
Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guilin 541006, China
4.
Bureau of Geology and Mineral Prospecting and Exploitation of Guangxi Zhuang Autonomous Region, Nanning 530023, China

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Corresponding author: LI Qiang, glqiangli@163.com

Received Date: 06 February 2024

Revised Date: 13 June 2024

Accepted Date: 06 July 2024

Publish Date: 31 July 2024

Abstract

Abstract

Glomalin-related soil protein (GRSP) secreted by arbuscular mycorrhizal fungi is widely distributed worldwide, has stable properties and is not easily decomposed, which is an important component of soil organic matter. Extracting high quality GRSP is important for in-depth research on organic carbon sinks in calcareous soil from karst areas. However, in previous studies, the mechanism of GRSP in organic matter could not be further studied due to low extraction yield, insufficient extraction and non-specific products. Therefore, the high extraction amount of GRSP is of great significance to explore the formation and stabilization mechanism of organic matter in calcareous soil. This experiment selected four types of calcareous soil from karst areas: black, brown, yellow and red calcareous soil. By using orthogonal experiments of temperature and time, the optimal extraction conditions for easily extractable glomalin-related soil protein (EE-GRSP) related to particulate organic matter (POM) and mineral-associated organic matter (MAOM) in calcareous soil were selected. The experimental results showed that the highest extraction amount of EE-GRSP was achieved when POM and MAOM were extracted at 123℃ and 80min. After application into the four types of calcareous soil, the EE-GRSP contents increased from 4.6% to 34.2%. The BRIEF REPORT is available for this paper at http://www.ykcs.ac.cn/en/article/doi/10.15898/j.ykcs.yk202402060015.
- easily extractable glomalin-related soil protein /
- optimization of extraction method /
- particulate organic matter /
- mineral-associated organic matter

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References

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