Determination of Magnesium Isotopic Composition of Plant Chlorophylls Based on HPLC and MC-ICP-MS

AN Zihan; ZHANG Hongyu; YIN Jiaxin; ZHANG Pan; YAO Min; HUANG Kangjun

doi:10.15898/j.ykcs.202208300161

2024 Vol. 43, No. 3

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Article navigation > Rock and Mineral Analysis > 2024 > 43(3): 476-486

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AN Zihan, ZHANG Hongyu, YIN Jiaxin, ZHANG Pan, YAO Min, HUANG Kangjun. Determination of Magnesium Isotopic Composition of Plant Chlorophylls Based on HPLC and MC-ICP-MS[J]. Rock and Mineral Analysis, 2024, 43(3): 476-486. doi: 10.15898/j.ykcs.202208300161

Citation:

AN Zihan, ZHANG Hongyu, YIN Jiaxin, ZHANG Pan, YAO Min, HUANG Kangjun. Determination of Magnesium Isotopic Composition of Plant Chlorophylls Based on HPLC and MC-ICP-MS[J]. Rock and Mineral Analysis, 2024, 43(3): 476-486. doi: 10.15898/j.ykcs.202208300161

Determination of Magnesium Isotopic Composition of Plant Chlorophylls Based on HPLC and MC-ICP-MS

1.
State Key Laboratory of Continental Dynamics, Department of Geology, Northwest University, Xi’an 710069, China
2.
State Key Laboratory of Isotope Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China

More Information

Corresponding author: HUANG Kangjun, hkj@nwu.edu.cn

Received Date: 30 August 2022

Revised Date: 31 January 2024

Accepted Date: 20 February 2024

Publish Date: 31 May 2024

Abstract

Abstract

The accurate determination of magnesium isotope values of chlorophyll a and chlorophyll b is particularly important for the study of magnesium biosynthesis pathways during chlorophyll formation. HPLC is required before MC-ICP-MS can be utilized, but HPLC lacks a method for separating chlorophylls. Therefore, it is necessary to develop a set of methods for separating plant chlorophyll a and chlorophyll b. The samples separated by this method are suitable for magnesium isotope determination by MC-ICP-MS. Based on the detection wavelength of 665nm and C18 column (7.6mm×250mm, 5μm), the HPLC conditions were optimized by three-factor and three-level orthogonal design to obtain the required parameters. The correlation coefficients of chlorophyll a and chlorophyll b standard curves in the concentration range of 5−50mg/L were greater than 0.9996, the detection limits were 0.40−1.09mg/L, the quantification limits were 1.22−3.31mg/L, the relative standard deviation was less than 8.10%, and the recoveries were 91.92%−111.11%. The magnesium isotope data of the isolated samples also showed that the column temperature of 25℃, flow rate of 1mL/min, and methanol-acetone (80∶20, V/V) mobile phase were reliable as HPLC separation conditions. The method established in this paper provides technical support for the separation of chlorophyll a and chlorophyll b in plants, and the separated samples can be used for magnesium isotope determination. The BRIEF REPORT is available for this paper at http://www.ykcs.ac.cn/en/article/doi/10.15898/j.ykcs.202208300161.
- magnesium isotope /
- standard-sample-bracketing method /
- high performance liquid chromatography /
- mobile phase /
- chlorophyll a /
- chlorophyll b

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References

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