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2023 Vol. 50, No. 6
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ZHU Ruijie, ZHOU Xuejun, ZHAN Tao, MA Yongfa, WANG Xu, DONG Junling, LIU Ling, YANG Fengtian, LI Dong, SHI Yujia, SU Yujuan. 2023. Analysis of microbial diversity and community structure in thermal waters from Lindian geothermal field, Heilongjiang Province[J]. Geology in China, 50(6): 1667-1677. doi: 10.12029/gc20210602003
Citation: ZHU Ruijie, ZHOU Xuejun, ZHAN Tao, MA Yongfa, WANG Xu, DONG Junling, LIU Ling, YANG Fengtian, LI Dong, SHI Yujia, SU Yujuan. 2023. Analysis of microbial diversity and community structure in thermal waters from Lindian geothermal field, Heilongjiang Province[J]. Geology in China, 50(6): 1667-1677. doi: 10.12029/gc20210602003
shu

Analysis of microbial diversity and community structure in thermal waters from Lindian geothermal field, Heilongjiang Province

  • 1.

    Key Laboratory of Groundwater Resources and Environment(Jilin University), Ministry of Education, Changchun 130021, Jilin, China

  • 2.

    Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun 130021, Jilin, China

  • 3.

    The Institute of Ecological Geology Survey and Research of Heilongjiang Province, Harbin 150030, Heilongjiang, China

    • Fund Project: Supported by the National Natural Science Foundation (Youth) (No.41202167)
More Information
  • Author Bio: ZHU Ruijie, male, born in 1998, master candidate, mainly engaged in geology and geothermal research; E-mail: zhurj20@mails.jlu.edu.cn
  • Corresponding author: SU Yujuan, female, born in 1980, senior engineer, mainly engaged in genesis, development and utilization of geothermal resources; E-mail: suyj15@mails.jlu.edu.cn
Received Date:  02 June 2021
Revised Date:  03 March 2022
Publish Date:  25 December 2023
    Abstract

  • This paper is the result of geothermal survey engineering.

     Objective

    Researches on microbial community in geothermal systems is key to indicate geochemical environment and evolution of thermal fluids. Previous researches have shown that the thermal water in Lindian geothermal field is highly reducing and is rich in methane. The objective of this paper is to characterize the microbial diversity and community structure of the thermal water.

     Methods

    A total of 9 thermal water samples were collected and tested during exploitation and non-exploitation periods, and the microbial diversity, community structure and functional genes of the samples were analyzed.

     Results

    The dominant bacterial genera in the thermal water is mainly Acinetobacter, which is different from most geothermal systems reported, but are similar to that of oil fields. The bacterial diversity of the thermal waters during the exploitation period and the non-exploitation period is significantly different, i.e., the bacterial diversity in the non-exploitation period is higher than that in the exploitation period, while the archaeal diversity is not affected significantly by exploitation. The dominant archaea are mainly Euryarchaeota methanogens. Functional gene prediction of archaea shows that methane production in Lindian geothermal field is dominated by hydrogenotrophic methanogenesis, and secondarily by disproportionation of methyl groups and reduction of methyl compounds with H2.

     Conclusions

    The microbial diversity and community structure of the thermal waters from Lindian geothermal field is relatively unique, which is related to the high content of organic matters and thermal water exploitation.
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