Simultaneous Analysis of Organochlorinated Pesticides and Polychlorinated Biphenyls from Animal Tissue using Gas Chromatography-Mass Spectrometry Combined with Accelerated Solvent Extraction

Ling TONG; Jia-jia YANG; Ni YAN; Shu-qi WU

2014 Vol. 33, No. 2

Article Contents

Article navigation > Rock and Mineral Analysis > 2014 > 33(2): 262-269

Next Article Previous Article

Ling TONG, Jia-jia YANG, Ni YAN, Shu-qi WU. Simultaneous Analysis of Organochlorinated Pesticides and Polychlorinated Biphenyls from Animal Tissue using Gas Chromatography-Mass Spectrometry Combined with Accelerated Solvent Extraction[J]. Rock and Mineral Analysis, 2014, 33(2): 262-269.

Citation:

Simultaneous Analysis of Organochlorinated Pesticides and Polychlorinated Biphenyls from Animal Tissue using Gas Chromatography-Mass Spectrometry Combined with Accelerated Solvent Extraction

1.
National Research Center for Geoanalysis, Beijing 100037, China
2.
School of Water Resources & Environment, China University of Geosciences (Beijing), Beijing 100083, China

Received Date: 17 July 2013

Accepted Date: 13 August 2013

Abstract

Abstract

The majority of fat and impurities in animal tissues influence the analysis result for low concentration organic compounds. To overcome this issue, the animal sample was extracted by accelerated solvent extraction (ASE) with methylene chloride/acetone (V:V=1:1), which reduced the co-extraction lipids during the extraction process. Gel Permeation Chromatography (GPC) combined with solid phase extraction (SPE) was applied to remove lipids from the sample solution. The GPC can remove most of the macromolecular lipids in samples, and the SPE with florisil can sufficiently reduce fat and small molecule impurity. The accuracy and sensitivity of the method can be improved by this pretreatment process. The compounds, including 17 organochlorinated pesticides and 7 indicator polychlorinated biphenyls in animal tissue, can be simultaneously analysed by Gas Chromatography Mass Spectrometry. The recoveries of 25 of the target compounds were in the range of 81.6%-113.4% and the method detection limits for dry samples were 1.02-3.59 ng/g. These results were superior to those of the national standard. This method improved the purification effect of animal samples, reduced the method detection limit, and enhanced the accuracy. It can be used to quickly and accurately determine the low content of persistent organic pollutants in animals.
- animal tissue /
- organochlorine pesticides /
- polychlorinated biphenyls /
- accelerated solvent extraction /
- Gel Permeation Chromatography /
- solid phase extraction /
- Gas Chromatography-Mass Spectrometry

FullText(HTML)

References

[1]	席英玉.动物性食品中有机氯农药多残留检测方法的研究[J].福建水产,2006(2):38-43. Google Scholar
[2]	张海秀.农业环境中有机氯农药污染现状及危害[J].科技信息,2009(14):548-549. doi: 10.3969/j.issn.1001-9960.2009.14.452 CrossRef Google Scholar
[3]	Liu X, Zhang G, Li J, Yu L L, Xu Y, Li X D, Kobara Y, Jones K C.Seasonal patterns and current sources of DDTs, chlordanes, hexachlorobenzene, and endosulfan in the atmosphere of 37 Chinese cities[J].Environmental Science & Technology, 2009, 43: 1316-1321. Google Scholar
[4]	Guo Y, Yu H Y, Zhang B Z, Zeng E Y. Persistent halogenated hydrocarbons in fish feeds manufactured in South China[J].Journal of Agricultural and Food Chemistry, 2009, 57: 3674-3680. doi: 10.1021/jf803868b CrossRef Google Scholar
[5]	王泰,张祖麟,黄俊,胡洪营,余刚,李发生.海河与渤海湾水体中溶解态多氯联苯和有机氯农药污染状况调查[J].环境科学,2007,28(4):730-735. Google Scholar
[6]	韩关根,徐盈,凌波,梁鹰,沈阿根.环境多氯联苯污染状况研究[J].卫生研究,2006,35(2):168-174. Google Scholar
[7]	宋杨,吴南翔,韩见龙,沈海涛,谭玉凤,丁刚强,项菊香,陶核,金顺亮.某电子垃圾拆解地鲫鱼和鸡蛋中二英和多氯联苯的污染状况研究[J].环境与健康,2011,28(4):328-331. Google Scholar
[8]	Qin Y Y, Leung C K M, Lin C K, Leung A O W, Wang H S, Giesy J P, Wong M H. Halogenated POPs and PAHs in Blood Plasma of Hong Kong Residents[J].Environmental Science & Technology,2001,45: 1630-1637. Google Scholar
[9]	Moliner-Martinez Y, Campíns-Falcó P, Molins-Legua C, Segovia-Martínezb L, Seco-Torrecillasb A. Miniaturized matrix solid phase dispersion procedure and solid phase microextraction for the analysis of organochlorinated pesticides and polybrominated diphenylethers in biota samples by gas chromatography electron capture detection[J].Journal of Chromatography A, 2009, 1216: 6741-6745. doi: 10.1016/j.chroma.2009.08.021 CrossRef Google Scholar
[10]	Rezaeia F, Hosseini M R M. New method based on combining ultrasonic assisted miniaturized matrix solid-phase dispersion and homogeneous liquid-liquid extraction for the determination of some organo-chlorinated pesticides in fish[J].Analytica Chimica Acta, 2011, 702: 274-279. doi: 10.1016/j.aca.2011.06.008 CrossRef Google Scholar
[11]	李君君,李力军,徐惠诚,赵增强,高彬,刘琨.动物源性食品中农药多残留检测前处理技术研究进展[J].中国食品卫生杂志,2012,24(4):403-407. Google Scholar
[12]	吴刚,赵珊红,俞春燕,王华雄,叶庆富,吴慧明.加速溶剂萃取-GPC气相色谱(μ-ECD)快速分析动物源性食品中多种电负性农药残留量[J].中国食品学报,2009,9(2):162-170. Google Scholar
[13]	Oukebdane K, Portet-Koltalo F N. Machoura, Dionnet F, Desbene P L. Comparison of hot Soxhlet and accelerated solvent extractions with microwave and supercritical fluid extractions for the determination of polycyclic aromatic hydrocarbons and nitrated derivatives strongly adsorbed on soot collected inside a diesel particulate filter[J].Talanta, 2010, 82:227-236. doi: 10.1016/j.talanta.2010.04.027 CrossRef Google Scholar
[14]	Chung S W C, Chen B L S.Determination of organochlorine pesticide residues in fatty foods: A critical review on the analytical methods and their testing capabilities[J].Journal of Chromatography A, 2011, 1218: 5555-5567. doi: 10.1016/j.chroma.2011.06.066 CrossRef Google Scholar
[15]	Beck J, Totsche K U, Kögel-Knabner I. A rapid and efficient determination of natural estrogens in soils by pressurised liquid extraction and gas chromatography-mass spectrometry[J].Chemosphere, 2008, 71: 954-960. doi: 10.1016/j.chemosphere.2007.11.062 CrossRef Google Scholar
[16]	Saito K, Sjodin A, Sandau C D, Davis M D, Nakazawa H, Matsuki Y, Patterson J D G. Development of a accelerated solvent extraction and gel permeation chromatography analytical method for measuring persistent organohalogen compounds in adipose and organ tissue analysis[J].Chemosphere, 2004, 57: 373-381. doi: 10.1016/j.chemosphere.2004.04.050 CrossRef Google Scholar
[17]	潘娟,华贤辉,商军.毛细管气相色谱法测定动物性食品中六六六和滴滴涕的残留量[J].中国兽药杂志,2009,43(3):46-49. Google Scholar
[18]	周萍萍,陈惠京,赵云峰,吴永宁,荫士安.动物性食品中持久性有机氯农药的残留分析[J].中国食品卫生杂志,2010,22(3):193-198. Google Scholar
[19]	佟玲,杨佳佳,吴淑琪,张玲金.沉积物样品中干扰物的去除及多种持久性有机污染物气相色谱分析[J].岩矿测试,2011,30(5):601-605. Google Scholar
[20]	曹忠波,高岩,贾宏新.凝胶渗透色谱和固相萃取净化气相色谱-质谱法检测动物性食品中26种有机氯农药残留[J].中国卫生检验杂志,2012,22(7): 1550-1555. Google Scholar
[21]	纪欣欣,石志红,曹彦忠,石利利,王娜,庞国芳.凝胶渗透色谱净化/液相色谱-串联质谱法对动物脂肪中111种农药残留量的同时测定[J].分析测试学报,2009,28(12):1433-1439. doi: 10.3969/j.issn.1004-4957.2009.12.017 CrossRef Google Scholar
[22]	吴刚,鲍晓霞,王华雄,俞春燕,吴慧明,叶庆富.加速溶剂萃取-凝胶渗透色谱净化-气相色谱快速分析动物源性食品中残留的多种有机磷农药[J].色谱,2008,26(5):577-582. Google Scholar
[23]	Wang H, Zhang J, Gao F, Yang Y, Duan H, Wu Y, Berset J D, Shao B. Simultaneous analysis of synthetic musks and triclosan in human breast milk by gas chromatography tandem mass spectrometry[J].Journal of Chromatography B, 2011, 879:1861-1869. doi: 10.1016/j.jchromb.2011.04.036 CrossRef Google Scholar
[24]	Helaleh M I H, Al-Rashdan A, Ibtisam A.Simultaneous analysis of organochlorinated pesticides (OCPs) and polychlorinated biphenyls (PCBs) from marine samples using automated pressurized liquid extraction (PLE) and Power Prep^TM clean-up[J].Talanta, 2012, 94: 44-49. doi: 10.1016/j.talanta.2012.02.031 CrossRef Google Scholar
[25]	Richter B E, Jones B A, Ezzell J L, Porter N L.Accelerated solvent extraction: A technique for sample preparation[J].Analytical Chemistry, 1996,68: 1033-1039. doi: 10.1021/ac9508199 CrossRef Google Scholar
[26]	Wu G, Bao X, Zhao S, Wu J, Han A, Ye Q.Analysis of multi-pesticide residues in the foods of animal origin by GC-MS coupled with accelerated solvent extraction and gel permeation chromatography cleanup[J].Food Chemistry, 2011, 126: 646-654. doi: 10.1016/j.foodchem.2010.10.105 CrossRef Google Scholar
[27]	程悦,贾倩楠,邹晓莉.加速溶剂萃取在农药检测中的应用[J].现代预防医学,2012,39(14):3627-3631. Google Scholar
[28]	Lang Y H, Cao Z M, Nie X H.Extraction of organo-chlorine pesticides in sediments using Soxhlet, ultrasonic and accelerated solvent extraction techniques[J]. Journal of Ocean University of China, 2005, 4(2): 173-176. doi: 10.1007/s11802-005-0012-8 CrossRef Google Scholar