The Disparity of Chain Length Distribution Patterns and Carbon Isotopic Compositions between Different Fatty Acid Purification Procedures

Jiang-tao ZHAO; Wei-guo LIU; Zhi-sheng AN

2013 Vol. 32, No. 1

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Article navigation > Rock and Mineral Analysis > 2013 > 32(1): 101-107

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Jiang-tao ZHAO, Wei-guo LIU, Zhi-sheng AN. The Disparity of Chain Length Distribution Patterns and Carbon Isotopic Compositions between Different Fatty Acid Purification Procedures[J]. Rock and Mineral Analysis, 2013, 32(1): 101-107.

Citation:

The Disparity of Chain Length Distribution Patterns and Carbon Isotopic Compositions between Different Fatty Acid Purification Procedures

1.
State Key Laboratory of Loess and Quaternary Geology, Institute of Earth Environment, Chinese Academy of Sciences, Xi′an 710075,China
2.
School of Chinese Academy of Sciences, Beijing 100049,China
3.
School of Human Settlement and Civil Engineering, Xi′an Jiaotong University, Xi′an 710049,China

More Information

Corresponding author: Wei-guo LIU, liuwg@loess.llqg.ac.cn

Received Date: 12 July 2012

Accepted Date: 27 August 2012

Abstract

Abstract

Saturated fatty acids and their isotopic composition are important proxies to reconstruct paleoenvironment and paleoclimate. There are several extraction and purification procedures of n-fatty acids based on different principles. However, a comparison test between different processes has not been reported. In this article, the agreement of fatty acid and its isotope compositions purified by different processes directly impacted on the proxies′ application, especially on the comparison of reconstruction results from different regions in global change research. Two common fatty acid purification procedures were used to purify lipids of fatty acid standards, plant and peat samples. For the standards, the recovery rates of these two procedures were all greater than 85%, which indicates that these two methods are reliable to process the n-fatty acids. However, for the plants and peats samples, Procedure 1 obtained a relatively large amount of shorter chain length n-fatty acids. According to our results, Procedure 2 obtained almost all of the free fatty acids, while Procedure 1 can obtain both the free and ester state n-fatty acids in the samples. Since the free state fatty acids and esters state fatty acids in the sediment can be transformed into each other, using Procedure 1 to analyze the total fatty acids in the sediment sample was more appropriate. Alternatively, we can also use the Procedure 2 after the ester-state fatty acids in the lipids were released as free state acids by saponification.
- fatty acids /
- distribution characteristics of chain length /
- carbon isotope composition /
- purification procedure of fatty acids

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References

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