| Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences | Host |
| Groundwater Science and Engineering Limited | Publish |
| Citation: |
Liu Ya-ci, Zhang Zhao-ji, Zhao Xin-yi, Wen Meng-tuo, Cao Sheng-wei, Li Ya-song. 2021. Arsenic contamination caused by roxarsone transformation with spatiotemporal variation of microbial community structure in a column experiment. Journal of Groundwater Science and Engineering, 9(4): 304-316. doi: 10.19637/j.cnki.2305-7068.2021.04.004
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Arsenic contamination caused by roxarsone transformation with spatiotemporal variation of microbial community structure in a column experiment
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Abstract
Arsenic contamination from roxarsone in livestock manure is common, and livestock manure continuously accumulates in the open environment. Evaluations of the environmental processes of As mobilization and transformation are critical for predicting the fate of As compounds after roxarsone degradation. In this study, spatiotemporal variations in As species and microbial community structure were characterized using laboratory column experiments with background soil collected from Yanggu County (northern Shandong Plain, China), a region of intense poultry production. Organic and inorganic arsenic were detected by high-performance liquid chromatography (HPLC) and HPLC with hydride generation atomic fluorescence spectrometry (HPLC-HG-AFS), respectively. High-throughput sequencing technology was used to describe microbial diversity. Results showed that roxarsone was transformed completely within 7 days, and As(Ⅲ) and As(Ⅴ) were the major degradation products. The concentration of As(Ⅲ) was much lower than that of As(Ⅴ). The As(Ⅲ) concentration increased significantly after Day 14, whereas the As(Ⅴ) concentration increased significantly after Day 84, indicating that As(Ⅲ) was initially produced. The microbial community structure changed significantly as roxarsone transformed into various As compounds. A critical and dominant bacterial strain, norank_f__Family_XVⅢ, was found to be related to the degradation of roxarsone into As(Ⅲ). This study improves our understanding of the fate of As species released from poultry litter to soil and groundwater, which is a threat to human health and environment.
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Keywords:
- Arsenic /
- Roxarsone /
- Spatiotemporal variation /
- Microbial community
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References
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Liu Ya-ci, Zhang Zhao-ji, Zhao Xin-yi, Wen Meng-tuo, Cao Sheng-wei, Li Ya-song. 2021. Arsenic contamination caused by roxarsone transformation with spatiotemporal variation of microbial community structure in a column experiment. Journal of Groundwater Science and Engineering, 9(4): 304-316. doi: 10.19637/j.cnki.2305-7068.2021.04.004
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Figure 1.
The particle size distribution of soil
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Figure 2.
Design of the one-dimensional soil column
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Figure 3.
Spatiotemporal variation in As(Ⅲ) and As(Ⅴ) concentrations
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Figure 4.
The variation in alpha diversity indices over time. CV: coefficient of variation.
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Figure 5.
Principal coordinate analysis (PCoA) of soil samples on genus level. Samples were named by sampling time (Day 7, 14, 28, 56, 84, or 112) and depth (d-1, d-2, d-3, d-4, d-5, d-6, and d-7 representing 5 cm, 15 cm, 25 cm, 35 cm, 45 cm, 55 cm, and 65 cm, respectively).
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Figure 6-1.
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Figure 6.
Relative microbial community abundance on genus level of different soil depths at six sampling times
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Figure 7.
The relative abundance of strain norank_f__Family_XVⅢ over time
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Figure 8.
Spearman correlation heatmap between dominant species and environmental factors. Red represents a positive correlation and blue represents a negative correlation; *: 0.01<P ≤0.05, **: 0.001<P ≤0.01, ***: P ≤0.001
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Figure 9.
Phylogenetic tree of the dominant strains on genus level (The bar chart on the right shows the abundance of strains at different sampling times.)