Influencing Factors of Dissolved Organic Carbon Isotope Determination in Water Samples of a Karst Area by Wet Oxidation Method

YANG Hui; WU Xia; YU Jianguo; TANG Wei; LAN Gaoyong

doi:10.15898/j.ykcs.202405130108

2024 Vol. 43, No. 6

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YANG Hui, WU Xia, YU Jianguo, TANG Wei, LAN Gaoyong. Influencing Factors of Dissolved Organic Carbon Isotope Determination in Water Samples of a Karst Area by Wet Oxidation Method[J]. Rock and Mineral Analysis, 2024, 43(6): 914-927. doi: 10.15898/j.ykcs.202405130108

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YANG Hui, WU Xia, YU Jianguo, TANG Wei, LAN Gaoyong. Influencing Factors of Dissolved Organic Carbon Isotope Determination in Water Samples of a Karst Area by Wet Oxidation Method[J]. Rock and Mineral Analysis, 2024, 43(6): 914-927. doi: 10.15898/j.ykcs.202405130108

Influencing Factors of Dissolved Organic Carbon Isotope Determination in Water Samples of a Karst Area by Wet Oxidation Method

1.
Institute of Karst Geology, Chinese Academy of Geological Sciences; Key Laboratory of Karst Dynamics, Ministry of National Resources & Guangxi Autonomous; Region/International Research Center on Karst under the Auspices of UNESCO, Guilin 541004, China
2.
Pingguo Guangxi, Karst Ecosystem, National Observation and Research Station, Pingguo, Guangxi 531406, China

More Information

Corresponding author: WU Xia, cugwuxia@126.com

Received Date: 13 May 2024

Revised Date: 28 September 2024

Accepted Date: 11 October 2024

Available Online: 29 October 2024

Publish Date: 31 December 2024

Abstract

Abstract

Due to the high content of HCO₃ ⁻ , low content of DOC and complex composition in the karst water, the current wet oxidation method for the determination of δ¹³C_DOC needs to be improved. The study examined the impact of different pure waters, headspace bottle atmospheric equilibrium methods, DOC content, and the removal of bicarbonate (HCO₃ ⁻ ) in water on δ¹³C_DOC values. The experimental results show significant differences in the ⁴⁴CO₂ peak areas produced by different blank pure waters, with the most prominent peak area being 3.8 times the smallest. Using a gas-tight syringe for atmospheric equilibrium enhances the sample signal strength and ensures the optimal internal precision of the sample test results. Acidifying the water samples to pH<3 with phosphoric acid eliminates the influence of HCO₃ ⁻ in karst waters. A nitrogen-blowing apparatus at a 250mL/min flow rate for 5−10min removes the free CO₂ present after acidification. Under the above experimental conditions, the deviation between the test results and the calibrated reference values for karst water samples with DOC content greater than 2mg/L is better than 0.40‰ after blank correction. The difference in the measured results of δ¹³C_DOC in karst water samples measured by the wet oxidation method compared to the total organic carbon analyzer-stable isotope mass spectrometer online method was less than 0.30‰. The BRIEF REPORT is available for this paper at http://www.ykcs.ac.cn/en/article/doi/10.15898/j.ykcs.202405130108.
- dissolved organic carbon /
- HCO₃ ⁻ /
- wet oxidation method /
- isotope mass spectrometry; blank sample

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References

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