Determination of Steroid Estrogens in Different Water Samples Using SPE-derivatization Coupled with GC-MS

Yuan-yuan WANG; Xiao-ming SONG; Yu-juan WEN; Adeel Muhammad; Yue-suo YANG; Wei SONG

doi:10.15898/j.cnki.11-2131/td.201705310092

2017 Vol. 36, No. 5

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Yuan-yuan WANG, Xiao-ming SONG, Yu-juan WEN, Adeel Muhammad, Yue-suo YANG, Wei SONG. Determination of Steroid Estrogens in Different Water Samples Using SPE-derivatization Coupled with GC-MS[J]. Rock and Mineral Analysis, 2017, 36(5): 519-528. doi: 10.15898/j.cnki.11-2131/td.201705310092

Citation:

Yuan-yuan WANG, Xiao-ming SONG, Yu-juan WEN, Adeel Muhammad, Yue-suo YANG, Wei SONG. Determination of Steroid Estrogens in Different Water Samples Using SPE-derivatization Coupled with GC-MS[J]. Rock and Mineral Analysis, 2017, 36(5): 519-528. doi: 10.15898/j.cnki.11-2131/td.201705310092

Determination of Steroid Estrogens in Different Water Samples Using SPE-derivatization Coupled with GC-MS

1.
Key Laboratory of Regional Environment and Eco-restoration(Shenyang University), Ministry of Education, Shenyang 110044, China
2.
Key Laboratory of Groundwater Environment and Resources(Jilin University), Ministry of Education, Changchun 130021, China
3.
Xinmin Oil Production Plant of Jilin Oilfield, China Petroleum and Natural Gas Co., LTD, Songyuan 138001, China

More Information

Corresponding author: Yue-suo YANG, yangyuesuo@jlu.edu.cn

Received Date: 31 May 2017

Revised Date: 30 June 2017

Accepted Date: 17 July 2017

Abstract

Abstract

In order to solve trace-level steroid estrogen pollution in groundwater and surface water, a SPE-GC-MS approach to determine five steroid estrogens (SEs), E1, 17α-E2, 17β-E2, EE2 and E3, by optimizing of solid phase extraction (SPE), derivatization conditions and the secondary purification process of complex samples has been developed. The results show that Oasis HLB column, ethyl acetate elution and derivatizing at 40℃ for 20 min can achieve the best results for extraction. Moreover, the Generik NAX column activated by methyl is suitable for the secondary purification of complex samples. The linear ranges of E1, 17α-E2, and 17β-E2 are 5-1000 ng/L, whereas those of EE2 and E3 are 10-1000 ng/L. The detection and quantitation limits are 2-3 ng/L and 6.5-10 ng/L, respectively. The standard solution added recoveries of water samples range from 80% to 120%. The relative standard deviations of daily peak areas in the SEs determination range from 6.8% to 10%. This method was used to determine the SEs pollution levels of waters from pond, river, groundwater and sewage treatment plant effluent and results show that this detection technique can be effectively applied to the identification and evaluation of estrogen risk in surface water and groundwater samples.
- water samples /
- steroid estrogens /
- Gas Chromatography-Mass Spectrometry /
- Solid Phase Extraction /
- derivatization

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References

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