WANG Teng, JIN Zhenjiang, GUO Jiayi, ZHANG Shuangshuang, CHENG Yaping, ZHANG Qin, LI JinCheng. Comparison of abundance of CO2 fixing microbes in paddy soil from karst and non-karst area[J]. Carsologica Sinica, 2018, (1): 74-80. doi: 10.11932/karst2017y58
Citation: |
WANG Teng, JIN Zhenjiang, GUO Jiayi, ZHANG Shuangshuang, CHENG Yaping, ZHANG Qin, LI JinCheng. Comparison of abundance of CO2 fixing microbes in paddy soil from karst and non-karst area[J]. Carsologica Sinica, 2018, (1): 74-80. doi: 10.11932/karst2017y58
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Comparison of abundance of CO2 fixing microbes in paddy soil from karst and non-karst area
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Abstract
oil samples were collected from paddy field in the Maocun karst experimental site to study the gene abundance of cbbLR1,cbbLG1 and cbbM,as the index of CO2 fixing bacteria in typical karst area,mixed area and non-karst area with the utilization of real-time quantitative PCR technology.The results showed that the gene abundances of cbbLG1 in karst area were generally higher than that in non-karst area,and the maximum value was 1.42× 109copies · g-1.The gene abundances of cbbLR1 and cbbM in mixed area were significantly higher than that in non-karst area,and the maximum values were 2.06 × 109 copies · g-1 and 3.35 × 107 copies · g-1.Correlation analysis showed that the abundance of cbbLG1 was significantly correlated with organic carbon mass fraction,total nitrogen mass fraction,and cation exchange capacity in the soil,which were significant factors that affect the gene abundance of cbbLG1.The abundance of the three cbbL genes had different sensitivity to pH,which was significantly positive correlated with the cbbLG1 gene and negatively correlated with the cbbM gene.
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